Biological Transformation Of Bacteria And Pglo Plasmid Dna

Abstract: Biotechnology and Genetic Engineering are highly involved with the genetic transformation of bacteria with the help of plasmid DNA. Genetic engineering ultimately alters genetic information using genetic material from another organism. The objective of the experiment was to accomplish genetic transformation using E.coli bacteria and pGLO plasmid DNA. PGLO carries an enzyme named ÃŽ ²-lactamase that offers resistance to the ampicillin antibiotic. Therefore, bacterial cells can grow and reproduce using pGLO. Four transformation plates containing E.coli were segregated into 2 groups of experimental and control groups. Two experimental plates comprised pGLO, but the control plates did not. One experimental plate contained ampicillin and pGLO. The second experimental plate consisted of ampicillin and arabinose along with pGLO. Contrarily, one control plate simply had ampicillin. The second control plate only stored E.coli cells. 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